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Updated: Mar 24, 2026
The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
Published on: March 13, 2014
A fatty acid profiling method using liquid chromatography-high resolution mass spectrometry for improvement of
Akiko Yagi1, Satoshi Miyanaga2, Rojeet Shrestha2
1Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Sapporo 060-0812, Japan; Kamiya Ladies Clinic, Kita-3, Nishi-2, Sapporo 060-0003, Japan.
Background:
Liquid chromatography-high resolution mass spectrometry (LC-HRMS) can be useful to improve in vitro fertilization (IVF). This study aims to find out an association between embryonic growth and embryonic uptake of free fatty acid (FFA) from culture media by using LC-HRMS.
Methods:
Embryos (n=55) from 15 couples undergoing IVF were studied. An embryo was cultivated for up to 6 days in a 20 μl-medium drop under mineral oil, and classified by a morphological grading system into the good-growth group (n=32; good quality blastocysts) and the poor-growth group (n=23; non-blastocysts). The control study was set up without embryo. Extracted ion chromatogram of FFAs was collected in negative-ion mode for each medium sample obtained after use.
Results:
The percent change from control to sample in mass area for docosahexaenoic acid showed a decrease in the good-growth group than that in the poor-growth group (p<0.05). Decrease in %change of docosahexaenoic acid might indicate proper embryonic growth. Similar but insignificant change was observed for other essential FFAs, but not for non-essential FFAs.
Conclusion:
The proposed metabolomic approach using LC-HRMS might be a powerful tool for non-invasive evaluation of embryonic growth.
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