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Updated: Mar 24, 2026

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
Published on: January 29, 2019
Intracellular Trafficking of AAV5 Vectors
Balaji Balakrishnan1,2, Giridhara R Jayandharan1,2
11 Department of Hematology, Christian Medical College and Hospital , Vellore, Tamil Nadu, India.
Abstract:
The present study was designed to visualize the cellular trafficking of adeno-associated virus (AAV) vectors in general and AAV5 serotype vectors in particular. We fluorescently labeled AAV5 wild-type and a mutant (S652A) virus and studied their infection process by live cell imaging and confocal microscopy. Our data demonstrate considerable difference in the ability of these vectors to reach the nuclear compartment within the first 6 hr after infection.

