Biophysical constraints of optogenetic inhibition at presynaptic terminals
Mathias Mahn1, Matthias Prigge1, Shiri Ron1
1Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Abstract:
We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Thus, the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.
More Related Videos
Related Concept Videos
Excitatory and Inhibitory Effects of Neurotransmitters
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Neurochemical Transmission: Sites of Drug Action


