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Draft Genome Sequence of Megasphaera sp. Strain DJF_B143, an Isolate from Pig Hindgut Unable to Produce Skatole

Xiaoqiong Li1, Ian P G Marshall2, Lars Schreiber2

  • 1Department of Animal Science, Aarhus University, Tjele, Denmark Xiaoqiong.Li@anis.au.dk.

Genome Announcements
|March 8, 2016
PubMed

Insights

Megasphaera species are key pig hindgut microbes. This study presents the genome of Megasphaera sp. DJF_B143, a strain that does not produce the boar taint compound skatole.

Area of Science:

  • Microbiology
  • Genomics
  • Animal Science

Background:

  • Megasphaera species are abundant in the pig hindgut.
  • These bacteria are known butyrate producers, influencing gut health.
  • Some Megasphaera isolates produce skatole, a compound responsible for boar taint.

Purpose of the Study:

  • To provide a draft genome sequence of a specific pig hindgut Megasphaera isolate.
  • To characterize an isolate that does not produce skatole, offering a contrast to known skatole-producing strains.
  • To contribute genomic resources for studying pig gut microbiota and boar taint.

Main Methods:

  • Isolation of Megasphaera sp. DJF_B143 from pig hindgut.
  • Whole-genome sequencing and assembly.
  • Bioinformatic analysis of the draft genome.

Main Results:

  • A 2.58-Mbp draft genome for Megasphaera sp. DJF_B143 was generated.
  • Genomic analysis confirmed the absence of key genes associated with skatole production.
  • This isolate represents a non-skatole producing strain within the Megasphaera genus.

Conclusions:

  • The genome of Megasphaera sp. DJF_B143 provides valuable insights into the genetic makeup of pig hindgut Megasphaera.
  • This non-skatole producing strain can serve as a reference for comparative genomics.
  • Further research can utilize this genome to understand the factors influencing skatole production in related species.

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