Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

25
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
25
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations01:15

Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations

317
Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...
317
Inhibitors of Bacterial Protein Synthesis01:25

Inhibitors of Bacterial Protein Synthesis

24
Aminoglycosides constitute a highly potent class of bactericidal antibiotics that exert their antimicrobial effects by targeting the bacterial ribosome, specifically disrupting protein synthesis. These polycationic molecules consist of amino-modified sugars linked via glycosidic bonds to an aminocyclitol core such as 2-deoxystreptamine or streptamine. Their strong positive charges facilitate tight binding to the negatively charged phosphate backbone of ribosomal RNA (rRNA), primarily at the 16S...
24
Drug Accumulation During Multiple Dosing: Intermittent IV Infusions01:24

Drug Accumulation During Multiple Dosing: Intermittent IV Infusions

334
Intermittent intravenous (IV) infusion is a method of drug administration where medications are delivered over short infusion periods followed by intervals of no drug delivery. This approach helps to prevent sustained high drug concentrations in the bloodstream, reducing the risk of adverse effects associated with prolonged exposure. Unlike continuous infusion, steady-state concentrations may not be achieved during a single dosing cycle but can be reached through repeated...
334
Therapeutic Drug Monitoring: Affecting Factors01:29

Therapeutic Drug Monitoring: Affecting Factors

324
Therapeutic Drug Monitoring (TDM) is the clinical practice of measuring specific drug levels in a patient's blood or body tissues to manage and optimize therapy. TDM is crucial for drugs with narrow therapeutic windows, like warfarin and phenytoin, where incorrect doses can lead to treatment failure or severe side effects. This monitoring ensures the dosage administered is within a safe and effective range. The factors affecting therapeutic drug monitoring include:Patient-Specific Factors:a.
324
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

60
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
60

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Slow-growing and fastidious uropathogens in hospitalized patients: an up-to-date review.

Future microbiology·2026
Same author

β-lactam susceptibility testing of ampicillin-susceptible non-faecalis enterococcal clinical isolates in France.

The Journal of antimicrobial chemotherapy·2026
Same author

Bacterial epidemiology of male urinary tract infections in France: An expert review.

Infectious diseases now·2026
Same author

Antibiotic penetration in the male urinary tract: a critical review of pharmacokinetic and pharmacodynamic evidence.

Infectious diseases now·2026
Same author

Updated guidelines for management of community-acquired urinary tract infections in adult men by the French infectious disease society (SPILF).

Infectious diseases now·2026
Same author

Revue de l'infirmiere·2026

Related Experiment Video

Updated: Mar 24, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
11:17

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

13.5K

Study on daptomycin use and implementation of an antimicrobial stewardship program.

Camille Castel1, Arnaud de La Blanchardière2, Jocelyn Michon2

  • 1Service de pharmacie, centre hospitalier universitaire de Caen, avenue de la Côte de Nacre, 14033, Caen Cedex 9, France. camille.castel@outlook.com.

International Journal of Clinical Pharmacy
|March 9, 2016
PubMed
Summary

Daptomycin use for Gram-positive infections was evaluated, revealing only 15% of prescriptions were appropriate. A multidisciplinary team developed a local consensus to improve the use of this important antibiotic.

Keywords:
Antimicrobial stewardshipBone and joint infectionDaptomycinEndocarditisFrancePrescribing audit

More Related Videos

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
11:28

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

29.9K

Related Experiment Videos

Last Updated: Mar 24, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
11:17

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

13.5K
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

3.5K
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
11:28

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

29.9K

Area of Science:

  • Infectious Diseases
  • Clinical Pharmacy
  • Antimicrobial Stewardship

Background:

  • Gram-positive pathogen infections, including MRSA and VRE, pose significant health challenges.
  • Daptomycin is an alternative for glycopeptide treatment failures but requires careful management due to cost and risks.
  • Increased daptomycin use since 2014 has sometimes extended beyond approved indications.

Purpose of the Study:

  • To analyze the appropriateness of daptomycin prescriptions in a university hospital setting.
  • To evaluate adherence to established guidelines for daptomycin use.

Main Methods:

  • A retrospective descriptive study was conducted from November 2013 to July 2014.
  • A multidisciplinary team (infectious diseases specialists, pharmacists, microbiologist) assessed prescription appropriateness.
  • Key indicators included treatment indication, prescribed dose, and co-administered antibiotics, based on 2011 IDSA recommendations.

Main Results:

  • Nineteen patients receiving daptomycin for at least two days were included.
  • Common indications were bone and joint infections (32%), endocarditis (26%), and bacteremia (26%).
  • Methicillin-resistant Staphylococcus aureus (MRSA) was the predominant pathogen (74%); only 15% of prescriptions met appropriateness criteria.

Conclusions:

  • A local consensus was developed due to a lack of recent guidelines for daptomycin use.
  • This multidisciplinary approach aims to optimize the utilization of daptomycin, an essential anti-MRSA agent.
  • Improved stewardship of reserved antibiotics like daptomycin is crucial for effective treatment of resistant infections.