Laser capture microdissection to identify septum-associated proteins in Aspergillus nidulans
Ying Zhang1, Ines Teichert2, Ulrich Kück2
1Karlsruhe Institute of Technology (KIT) South Campus, Institute for Applied Biosciences Dept. of Microbiology, Hertzstrasse 16, D-76187 Karlsruhe, Germany.
Abstract:
To spatially resolve genetic differences at the cellular level, the laser-capture microdissection technique was developed. With this method cells can be cut from tissues with a laser beam and analyzed for DNA, RNA or protein composition. Here we adapted the technique to isolate septal microtubule-organizing center (MTOC)-associated proteins in Aspergillus nidulans About 3000 septa were collected and subjected to peptide fingerprinting by mass-spectrometric analysis. We identified the microtubule polymerase AlpA and found it interacts with ApsB specifically at sMTOCs, suggesting that AlpA might be involved in the assembly or the functioning of this protein complex.
