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Related Experiment Videos

Calcium: an important second messenger in mast cells.

C Fewtrell1, F C Mohr, T A Ryan

  • 1Department of Pharmacology, Cornell University, Ithaca, NY 14853.

Ciba Foundation Symposium
|January 1, 1989
PubMed
Summary

Calcium is crucial for mast cell secretion, originating from internal stores and sustained by plasma membrane influx. Other messengers also play a role, highlighting the need for single-cell studies.

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Area of Science:

  • Immunology
  • Cell Biology
  • Calcium Signaling

Background:

  • Controversy exists regarding the mechanisms of intracellular calcium elevation in mast cells upon IgE receptor aggregation by antigen.
  • The role of calcium in initiating mast cell secretion has been questioned.

Purpose of the Study:

  • To clarify the role of calcium and other intracellular messengers in mast cell stimulus-secretion coupling.
  • To investigate the source and maintenance of intracellular calcium increase in antigen-stimulated mast cells.
  • To explore the nature of the calcium permeability pathway in mast cells.

Main Methods:

  • Utilized a mast cell line for studying stimulus-secretion coupling.
  • Employed digital imaging microscopy for single-cell measurements of antigen-stimulated mast cell responses.

Main Results:

  • Confirmed the importance of calcium in mast cell physiological responses, alongside other intracellular messengers.
  • Demonstrated that intracellular calcium increase originates from internal stores but requires plasma membrane influx for sustained elevation.
  • Observed significant oscillations and spatial gradients of intracellular calcium in individual cells.

Conclusions:

  • Calcium is essential for mast cell secretion, with contributions from both intracellular stores and plasma membrane influx.
  • The precise calcium channel involved in mast cells remains to be identified.
  • Single-cell measurements are vital for understanding the complex interplay between IgE receptor activation, calcium dynamics, and exocytosis.

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