Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

USA millennial and non-millennial beef consumers perception of beef, pork, and chicken.

Meat science·2024

Same author

National Beef Quality Audit-2016: Transportation, mobility, and harvest-floor assessments of targeted characteristics that affect quality and value of cattle, carcasses, and by-products.

Translational animal science·2020

Same author

National Beef Quality Audit-2016: Phase 1, Face-to-face interviews.

Translational animal science·2020

Same author

National Beef Quality Audit-2016: Transportation, mobility, live cattle, and carcass assessments of targeted producer-related characteristics that affect value of market cows and bulls, their carcasses, and associated by-products.

Translational animal science·2020

Same author

Young children, adolescent girls and women with type 1 diabetes are more overweight and obese than reference populations, and this is associated with increased cardiovascular risk factors.

Diabetic medicine : a journal of the British Diabetic Association·2019

Same author

An intravenous insulin protocol designed for pregnancy reduces neonatal hypoglycaemia following betamethasone administration in women with gestational diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2018

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Published on: May 12, 2020

Improved sensitivity for determining thiobarbituric acid reactive substances in ground beef.

C R Kerth¹, C W Rowe²

¹Department of Animal Sciences, Texas A&M University, College Station, TX 77843, United States.

|March 9, 2016

Summary

This study compared oxidation in ground beef from grass-fed and grain-fed cattle using various packaging. A modified TBARS method improved detection of differences, offering a more efficient assay for researchers.

Keywords:

Beef Grain-fed Grass-fed MAP Oxidation Packaging TBARS

More Related Videos

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Published on: September 20, 2016

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Related Experiment Videos

Last Updated: Mar 24, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Published on: May 12, 2020

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Published on: September 20, 2016

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Area of Science:

Food Science
Meat Science
Analytical Chemistry

Background:

Oxidation impacts ground beef quality and shelf-life.
Differentiating between grass-fed and grain-fed beef requires sensitive analytical methods.
Packaging significantly influences lipid oxidation in meat products.

Purpose of the Study:

To evaluate the impact of grass-fed vs. grain-fed origins on ground beef oxidation.
To assess the effectiveness of four different packaging types on oxidation.
To compare the sensitivity and variability of a modified Thiobarbituric Acid Reactive Substances (TBARS) assay versus the original method.

Main Methods:

Ground beef samples were created using six different ratios of grass-fed to grain-fed beef.
Four packaging treatments were applied: high oxygen (HO), low oxygen (LO), carbon monoxide (CO), and overwrap (OV).
Lipid oxidation was measured using both a modified and original TBARS extraction method.

Main Results:

The modified TBARS method demonstrated higher sensitivity in detecting differences between treatments compared to the original method.
The modified TBARS assay exhibited less variability in results.
Significant differences in oxidation were observed across the various grass-fed/grain-fed ratios and packaging types.

Conclusions:

The modified TBARS method provides a more effective and less labor-intensive assay for researchers studying lipid oxidation in ground beef.
This research offers insights into managing oxidation in ground beef based on animal diet and packaging.
Optimizing packaging and understanding feed source are crucial for maintaining ground beef quality.