Related Experiment Video
Updated: Mar 24, 2026
06:55
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
Published on: September 24, 2015
8.8K
A simple, accurate and universal method for quantification of PCR.
Nicky Boulter1, Francia Garces Suarez2, Stephen Schibeci3
1Accugen Pty Ltd, 11 Julius Avenue, North Ryde, NSW, 2113, Australia. nicolab@accugen.com.au.
BMC Biotechnology
|March 10, 2016
Summary
AccuCal offers accurate gene expression quantification using quantitative real-time PCR (qPCR). This universal calibrator overcomes traditional method limitations for reliable experimental conclusions.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Gene expression research is crucial for understanding biological processes.
- Quantitative real-time PCR (qPCR) is a key method for gene expression analysis.
- Conventional qPCR quantification methods can be inaccurate or labor-intensive.
Purpose of the Study:
- To introduce AccuCal, a novel universal calibrator for qPCR.
- To provide an accurate and user-friendly solution for gene expression quantification.
- To overcome the limitations of existing qPCR quantification methodologies.
Main Methods:
- Developed and validated AccuCal as a universal calibrator.
- Utilized AccuCal with both dye- and probe-based qPCR detection methods.
- Assessed accuracy over a dynamic range of ≥10(5) copies for amplicons up to 500 bp.
Main Results:
- AccuCal provides accurate absolute quantification for all genes of interest.
- Demonstrated accuracy across a wide dynamic range (≥10(5) copies).
- Successfully used with various qPCR detection chemistries and amplicon sizes (up to 500 bp).
Conclusions:
- AccuCal offers a superior alternative to traditional qPCR quantification methods.
- Enables objective experimental conclusions by circumventing known qPCR biases.
- Proposes AccuCal as a replacement for conventional PCR quantification techniques.
