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Updated: Mar 24, 2026
![Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63025.jpg&w=3840&q=50)
Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
Tryptophan-induced pathogenesis of breast cancer
Zhu-Gen Cao1, Xiao-Bing Qin2, Fang-Fang Liu3
1Department of Oncology, Si'hong County People's Hospital, Si'hong, Jiangsu, 223900, P.R. China.
Background:
The pathogenesis of breast cancer remains unclear.
Aims:
To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.
Methods:
Patients with breast cancers were enrolled in our hospital between year January 1(st), 2013 and December 31(st), 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.
Results:
Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.
Conclusion:
The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.
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