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High-performance liquid chromatography separation of nikkomycins X and Z
P Engel1, H C Dischinger, A H Ullah
1Southern Regional Research Laboratory, USDA, New Orleans, Louisiana 70124.
Preparative Biochemistry
|January 1, 1989
Abstract:
A reversed-phase, C-18 HPLC method for separation, with baseline resolution, of the chitin synthase inhibitors nikkomycin X and Z is described. This permits, for the first time, satisfactory identification of nikkomycin X and Z contained in a mixture. The use of 30 mM ammonium formate (pH 4.7) containing the ion-pair agent heptanesulfonic acid (1 mM) was critical for the successful separation of these fungicides.