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Linear chromosome of Borrelia burgdorferi
C Baril1, C Richaud, G Baranton
1Unité des Leptospires, Institut Pasteur, Paris.
Research in Microbiology
|October 1, 1989
Abstract:
The DNA organization of several European and American isolates of Borrelia burgdorferi, the aetiological agent of Lyme disease, was analysed in pulse-field agarose gel electrophoresis. The results of in situ cell lysis in agarose plugs demonstrated a unique arrangement for the DNA of this spirochete. The chromosome of Borrelia behaved as a eukaryotic linear chromosome with a size of around 1,000 kb. The genome also comprised several circular and linear plasmids which varied in size from 15 to 60 kb.