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Updated: Mar 24, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment
Sarah F Mitchell1, Roy Parker2
1Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado Boulder, Jennie Smoly Caruthers Biotech Bldg., 3415 Colorado Avenue, Boulder, CO, 80303, USA. sarah.f.mitchell@colorado.edu.
Abstract:
The maturation, localization, stability, and translation of messenger RNAs (mRNAs) are regulated by a wide variety of mRNA-binding proteins. Identification of the complete set of mRNA-binding proteins is a key step in understanding the regulation of gene expression. Herein, we describe a method for identifying yeast mRNA-binding proteins in a systematic manner using UV crosslinking, purification of polyA(+) mRNAs under denaturing conditions, and mass spectrometry to identify covalently bound proteins.

