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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
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Protein extracts from cultured cells contain nonspecific serum albumin
Masatsugu Miyara1,2, Kanae Umeda1, Keishi Ishida1,2
1a Graduate School of Biomedical and Health Sciences , Hiroshima University , Hiroshima , Japan.
Bioscience, Biotechnology, and Biochemistry
|March 12, 2016
Abstract:
Serum is an important component of cell culture media. The present study demonstrates contamination of intracellular protein extract by bovine serum albumin from the culture media and illustrates how this contamination can cause the misinterpretation of western blot results. Preliminary experiments can prevent the misinterpretation of some experimental results, and optimization of the washing process may enable specific protein detection.
