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Updated: Aug 10, 2026

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
Published on: January 2, 2018
[Histologic and light-microscopic cytomorphometric studies of the tissue reparative processes in complete wound
Abstract:
The authors carried out comparative histological and light microscopic cytomorphometric studies on tissue material, obtained during fixation of secondary sutures on complete wound dehiscences after obstetric and gynecological operations, prepared for therapy only by helium-neon laser irradiation. Densities of the power of helium-neon laser irradiation 90,70 an and 50 mW/cm2 were investigated at exposition time of the field of 1.5 min. The number of irradiations was 7-9 (after applied density of the power of 90, and 70 mW/cm2) and 9-12 (after irradiation with densities of the power of 50 mW/cm2). Histological and light microscopic cytomorphometric studies showed that the applied density of the power of laser irradiation of 90 mW/cm2 stimulated most actively the processes of tissue repair in complicated operative wounds. A considerable slowing of the processes of tissue repair was established after irradiation with densities of the power of 50 mW/cm2.

