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Updated: Mar 24, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Effects of static magnetic fields on bone regeneration of implants in the rabbit: micro-CT, histologic, microarray,
Eun-Cheol Kim1, Richard Leesungbok2, Suk-Won Lee2
1Department of Oral and Maxillofacial Pathology, School of Dentistry and Institute of Oral Biology, Kyung Hee University, Seoul, Korea.
Objectives:
The aim of this study was to investigate the effects of static magnetic fields (SMFs) on bone regeneration around titanium implants by μCT, histologic analysis, microarrays, and quantitative real-time PCR (qRT-PCR).
Materials And Methods:
Neodymium magnets provided the source of SMFs, the specimens were grade 5 titanium implants, and the animals were twenty-seven adult male New Zealand white rabbits. These implants were divided into six groups according to the presence of a magnet and predetermined healing period (1, 4, and 8 weeks). Each group comprised six specimens for μCT (n = 6) and histologic examination, and three specimens (n = 3) for microarrays and qRT-PCR, yielding a total of 54 specimens.
Results:
The μCT data showed that SMFs increased bone volume fraction (bone volume/total volume, BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th). Histologic observation indicated that SMFs promoted new bone formation and direct bony contact with implants. Microarray analysis identified 293 genes upregulated (>twofold) in response to SMFs. The upregulated genes included extracellular matrix (ECM)-related genes (COL10A1, COL9A1, and COL12A1) and growth factor (GF)-related genes (CTGF and PDGFD), and the upregulation was confirmed by qRT-PCR. Gene Ontology (GO) and pathway analysis revealed the involvement of the mitogen-activated protein kinase (MAPK), Wnt, and PPAR-gamma signaling pathways in implant healing.
Conclusions:
μCT, histology, microarrays, and real-time PCR indicate that SMFs could be an effective approach to improving bone regeneration around dental implants.
