Related Experiment Video
Updated: Jan 26, 2026

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Frapid: achieving full automation of FRAP for chemical probe validation
Clarence Yapp1, Catherine Rogers2, Pavel Savitsky3
1Target Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK; Botnar Research Centre, NDORMS, University of Oxford, Oxford, OX3 7LD, UK.
Abstract:
Fluorescence Recovery After Photobleaching (FRAP) is an established method for validating chemical probes against the chromatin reading bromodomains, but so far requires constant human supervision. Here, we present Frapid, an automated open source code implementation of FRAP that fully handles cell identification through fuzzy logic analysis, drug dispensing with a custom-built fluid handler, image acquisition & analysis, and reporting. We successfully tested Frapid on 3 bromodomains as well as on spindlin1 (SPIN1), a methyl lysine binder, for the first time.
Related Concept Videos
Reliability and Validity
Chemical Reactions
Chemical Reactions Rearrange Atoms into New Substances
A chemical reaction takes starting materials—the reactants—and changes them...
Types of Chemical Bonds
Chemical Formulas
Chemical Equations
Data Validation
Nursing assessment guides are generally based on holistic models rather than medical...

