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Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments
Lukas Kontenis1, Masood Samim2, Abiramy Karunendiran3
1Department of Physics and Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto, ON, M5S 1A7, Canada; Department of Chemical and Physical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Abstract:
The experimental implementation of double Stokes Mueller polarimetric microscopy is presented. This technique enables a model-independent and complete polarimetric characterization of second harmonic generating samples using 36 Stokes parameter measurements at different combinations of incoming and outgoing polarizations. The degree of second harmonic polarization and the molecular nonlinear susceptibility ratio are extracted for individual focal volumes of a fruit fly larva wall muscle.
