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Updated: Mar 24, 2026

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Genome-wide RNA-seq and ChIP-seq reveal Linc-YY1 function in regulating YY1/PRC2 activity during skeletal myogenesis.

Kun Sun1, Liang Zhou2, Yu Zhao2

  • 1Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong, China; Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, China.

Genomics Data
|March 17, 2016
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Summary

Researchers discovered Linc-YY1, a novel long non-coding RNA (lncRNA), crucial for skeletal muscle development. Linc-YY1 promotes myogenesis by interacting with the Yin Yang 1 transcription factor.

Keywords:
C2C12 cellsChIP-seqLinc-YY1MyogenesisRNA-seq

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Long non-coding RNAs (lincRNAs) roles in skeletal myogenesis remain largely unexplored.
  • Understanding lincRNA involvement is key to deciphering muscle development regulatory networks.

Purpose of the Study:

  • To discover and functionally annotate novel lincRNAs in skeletal muscle.
  • To elucidate the role of Linc-YY1 in skeletal myogenesis.

Main Methods:

  • Whole transcriptome shotgun sequencing (RNA-seq) for lincRNA identification.
  • Bioinformatics analysis for high-confidence novel lincRNA discovery.
  • Genome-wide approaches including ChIP-seq to determine Linc-YY1 function.

Main Results:

  • Identification of Linc-YY1, a novel lincRNA originating from the YY1 transcription factor promoter.
  • Demonstration that Linc-YY1 promotes myogenesis.
  • Evidence of Linc-YY1 associating with YY1 and regulating YY1/PRC2 transcriptional activity.

Conclusions:

  • Linc-YY1 is a novel and important regulator of skeletal myogenesis.
  • Linc-YY1 functions in trans to modulate YY1/PRC2 transcriptional activity, promoting muscle development.