[Application of Rapid PCR Technology on Authentication of Species in Panax Genus]

To establish an accurate, rapid and efficient method for authentication of Panax species by using PCR amplification of specific alleles.

The samples of Panax species were collected for extracting the total DNA. matK sequence from the Panax species was amplified by PCR and sequenced directionally, and then aligned by using Clustal W. Specific primers were designed and amplified by two-steps PCR amplification method.

By optimizing the denatured and annealing temperature and time, cycle numbers, the rapid PCR methods for authentication of Panax species were established respectively. When SYBR Green I was added in the PCR product, strong green fluorescence was visualized under 365 nm UV lamp whereas adulterants were not.

The rapid PCR method can identify the Panax species rapidly. This study provides the technical support for authentication of Chinese medicinal materials.