Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Centrifugation

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparing the i31-SLNB and the MIA Nomogram for Sentinel Lymph Node Biopsy Positivity Prediction in Cutaneous Melanoma: A Prospective Cohort Analysis.

Dermatology and therapy·2026

Same author

Ligation-assisted target recycling for DNA nanoswitch biosensors.

bioRxiv : the preprint server for biology·2026

Same author

Aptamer-based DNA nanoswitches for multiplexed protein detection.

bioRxiv : the preprint server for biology·2026

Same author

Complex intervention to improve empathy within maternity services: a mixed methods feasibility study with pilot evaluation.

BMJ open quality·2026

Same author

Paranemic Cohesion of DNA under Isothermal Conditions.

JACS Au·2026

Same author

Biomimetic Constructs for Achilles Tendon Regeneration and their Translation to Human Medicine.

Tissue engineering and regenerative medicine·2026

Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026

Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026

Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026

Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026

Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026

Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

Multiplexed single-molecule force spectroscopy using a centrifuge.

Darren Yang¹, Andrew Ward^2,3, Ken Halvorsen^2,3,4

¹School of engineering and applied sciences, Harvard University, Cambridge, Massachusetts 02138, USA.

Nature Communications

|March 18, 2016

Summary

This summary is machine-generated.

We developed a miniature centrifuge force microscope (CFM) for high-throughput single-molecule studies. This novel approach enhances reliability and allows for distinguishing molecular populations through force measurements.

More Related Videos

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Related Experiment Videos

Last Updated: Mar 24, 2026

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers

Published on: July 25, 2012

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Area of Science:

Biophysics
Molecular Biology
Nanotechnology

Background:

Single-molecule force spectroscopy is crucial for understanding molecular interactions.
Existing methods can be limited in throughput and operational simplicity.
Characterizing molecular heterogeneity requires sensitive and reliable techniques.

Purpose of the Study:

To introduce a novel miniature centrifuge force microscope (CFM) for high-throughput single-molecule experiments.
To demonstrate the CFM's capability for high-resolution particle tracking and broad force range.
To showcase the CFM's utility in characterizing molecular population heterogeneity.

Main Methods:

Repurposing a benchtop centrifuge into a miniature CFM.
Utilizing DNA nanoswitches for repeated force interrogation of single molecular pairs.
Implementing spatiotemporally multiplexed experiments for data collection.
Employing per-molecule statistics for noise reduction and population analysis.

Main Results:

Achieved high-throughput and reliable single-molecule force measurements.
Demonstrated a large force range and precise temperature control.
Collected 1,863 bond rupture statistics from 538 molecular pairs in a single experiment.
Successfully distinguished two populations of DNA zippers using the CFM.

Conclusions:

The miniature CFM offers a powerful, user-friendly platform for advanced single-molecule studies.
The system significantly increases throughput and reliability in force spectroscopy.
The CFM enables detailed characterization of molecular heterogeneity and population dynamics.