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Related Experiment Videos

[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

P Ia Arenkov, E N Zhmareva, V A Berezin

    Ukrainskii Biokhimicheskii Zhurnal (1978)
    |November 1, 1989
    PubMed
    Summary

    A novel enzyme-linked immunoassay enhances antigen immobilization using membrane technology for improved sensitivity. This modified assay offers reusable membranes and stable storage, enabling reliable immunoglobulin G detection.

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    A novel method for evaluation of carbohydrate-binding activity: enzyme-linked carbohydrate-binding assay (ELCBA).

    Biochemistry and molecular biology international·1994

    Area of Science:

    • Biochemistry
    • Immunology
    • Analytical Chemistry

    Context:

    • Conventional enzyme-linked immunosorbent assay (ELISA) methods have limitations in antigen immobilization.
    • Membrane technology offers advantages for solid-phase antigen attachment in immunosensing.
    • Developing robust and reusable immunoassay platforms is crucial for diagnostics.

    Purpose:

    • To develop a modified enzyme-linked immunoassay (ELISA) by integrating membrane technology for antigen immobilization.
    • To combine the benefits of membrane-based antigen immobilization with conventional ELISA principles.
    • To establish a stable and repeatable immunoassay for quantifying immunoglobulin G (IgG) concentrations.

    Summary:

    • A novel enzyme-linked immunoassay (ELISA) modification utilizes nitrocellulose and polypropylene membranes for antigen immobilization.

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  • Purified rabbit immunoglobulin G (IgG) is immobilized as the first layer, enabling competitive immunoassay detection within a 1-1000 ng/ml range.
  • The immobilized antigen membranes demonstrate reusability after glycine buffer treatment and stability for up to 6 months when refrigerated.

    • Impact:

    • This modified immunoassay offers a reusable and stable platform for antigen detection, reducing assay costs and improving efficiency.
    • The method enhances antigen immobilization, potentially leading to increased assay sensitivity and reliability.
    • Provides a foundation for developing improved diagnostic tools utilizing membrane-based immunoassay technologies.