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E-Cadherin Facilitates Protein Kinase D1 Activation and Subcellular Localization
Zhuo Li1,2, Chuanyou Zhang2, Li Chen2
1The First Affiliated Hospital of China Medical University, Shenyang, China.
Journal of Cellular Physiology
|March 19, 2016
Summary
E-cadherin binding to Protein Kinase D 1 (PKD1) activates it independently of protein kinase C (PKC). This novel mechanism suggests E-cadherin loss in cancer metastasis may alter PKD1 signaling and cell behavior.
Area of Science:
- Cell Biology
- Molecular Signaling
- Cancer Research
Background:
- Protein Kinase D 1 (PKD1) regulates cell growth, differentiation, adhesion, and motility.
- PKD1 activation typically involves diacylglycerol (DAG) binding and subsequent phosphorylation by protein kinase C (PKC) at cellular membranes.
Purpose of the Study:
- To identify novel regulatory mechanisms of PKD1 activation.
- To investigate the role of E-cadherin in PKD1 signaling.
- To explore the implications of E-cadherin-PKD1 interaction in cancer metastasis and cell function.
Main Methods:
- Investigated physical binding between E-cadherin and PKD1.
- Utilized artificial membrane targeting to assess PKD1 activation.
- Examined PKD1 activation and substrate specificity in the presence and absence of E-cadherin.
- Performed knockdown of PKD1 in A549 lung epithelial cells to observe cellular changes.
Main Results:
- E-cadherin physically binds to PKD1, causing its redistribution.
- Membrane localization of PKD1 is sufficient for its activation, independent of PKC.
- E-cadherin dynamically regulates PKD1 activation by Bryostatin 1 and its substrate specificity.
- PKD1 knockdown induced epithelial-mesenchymal transition (EMT), altered cell migration, and affected drug resistance in A549 cells.
Conclusions:
- E-cadherin represents a novel regulator of PKD1 activation.
- Loss of E-cadherin during metastasis may lead to altered PKD1 signaling and diverse cellular functions.
- This study expands understanding of PKD1 and E-cadherin signaling, potentially explaining PKD1's varied roles in different cell types.
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