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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

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Color-Coded Super-Resolution Small-Molecule Imaging.

Paolo Beuzer¹, James J La Clair^2,3, Hu Cang⁴

¹Waitt Advanced Biophotonics Center, The Salk Institute for Biological Sciences, 10010 N Torrey Pines Road, La Jolla, CA, 92037, USA.

Chembiochem : a European Journal of Chemical Biology

|March 21, 2016

Summary

This summary is machine-generated.

This study introduces a novel microscopy system for tracking small molecules in live cells and then performing super-resolution imaging. This advanced technique enhances understanding of how small molecules move within cells and their mechanisms of action.

Keywords:

drug discovery microscopy mode of action natural products super-resolution imaging

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Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Area of Science:

Cell biology
Microscopy
Pharmacology

Background:

Super-resolution microscopy, developed in 1994, has mainly visualized cellular structures like proteins, DNA, and sugars.
Tracking small molecules in live cells at high resolution has been a significant challenge.

Purpose of the Study:

To develop a novel microscopy system for monitoring exogenous small molecules in live cells.
To enable subsequent super-resolution imaging for detailed mechanistic studies.

Main Methods:

Utilizing a dual-mode system combining low-resolution live-cell imaging with high-resolution fixed-cell imaging.
Employing stochastic optical reconstruction microscopy (STORM) for super-resolution analysis.

Main Results:

Successfully monitored the localization of exogenous small molecules in live cells.
Achieved super-resolution imaging of small molecule trafficking pathways.

Conclusions:

The developed system offers a powerful new approach to study subcellular trafficking of small molecules.
Provides insights into the mode and mechanism of action of exogenous small molecules.