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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
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Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.

Christian G Roessler1, Rakhi Agarwal2, Marc Allaire1

  • 1Photon Sciences Division, Brookhaven National Laboratory, Upton, NY 11973-5000, USA.

Structure (London, England : 1993)
|March 22, 2016
PubMed
Summary
This summary is machine-generated.

Novel acoustic injection systems deliver crystal-containing droplets for X-ray free-electron laser (XFEL) experiments. This synchronized droplet delivery maximizes X-ray pulse interactions, improving macromolecular crystallography data collection for various samples.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • X-ray free-electron lasers (XFELs) offer high-intensity X-ray pulses for macromolecular crystallography.
  • Current methods face challenges synchronizing sample delivery with the short XFEL pulse duration.

Purpose of the Study:

  • To develop and validate novel acoustic injection systems for on-demand droplet delivery to XFELs.
  • To improve the efficiency and success rate of X-ray diffraction data collection.

Main Methods:

  • Two acoustic injection systems utilizing focused sound waves were designed.
  • Droplets (picoliter to nanoliter) containing microcrystals were ejected from microplates.
  • Droplet delivery was synchronized with the XFEL pulse scheme.

Main Results:

  • Up to 88% of droplets intersected with X-ray pulses.
  • Crystal structures of lysozyme, thermolysin, and stachydrine demethylase (Stc2) were determined.
  • The crystal hit ratio correlated with microcrystal slurry concentration.

Conclusions:

  • Acoustic injection systems enable efficient, synchronized sample delivery for XFEL crystallography.
  • These methods are applicable to a range of samples, including rare ones.
  • The technology enhances data collection for structural biology research.