Related Experiment Video

Updated: Mar 23, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

12.2K

CD97/ADGRE5 Inhibits LPS Induced NF-κB Activation through PPAR-γ Upregulation in Macrophages

Shuai Wang1, Zewei Sun1, Wenting Zhao1

  • 1Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China.

Mediators of Inflammation
|March 22, 2016
PubMed

Insights

CD97 protein suppresses lipopolysaccharide (LPS)-induced NF-κB activation and TNF-α secretion in macrophages, partly by upregulating the anti-inflammatory transcription factor PPAR-γ.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD97/ADGRE5, an EGF-TM7 receptor family member, is expressed on leukocytes and influences inflammatory responses.
  • Its role in lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-κB) activation in macrophages remains unexplored.

Purpose of the Study:

  • To investigate the role of CD97 in LPS-induced NF-κB activation and inflammatory factor production in macrophages.
  • To elucidate the involvement of PPAR-γ in CD97-mediated regulation of NF-κB signaling.

Main Methods:

  • Assessed CD97 expression in LPS-treated human primary macrophages.
  • Manipulated CD97 levels (knockdown/overexpression) and analyzed NF-κB and TNF-α.
  • Utilized immunofluorescence staining to examine p65 and PPAR-γ expression.
  • Employed CD97 knockout THP-1 cells to confirm findings.

Main Results:

  • CD97 overexpression inhibited LPS-induced p65 nuclear translocation and TNF-α secretion.
  • CD97 manipulation affected PPAR-γ expression, suggesting its involvement in the pathway.
  • CD97 knockout cells showed decreased LPS-induced NF-κB activation.
  • CD97 was found to promote PPAR-γ expression.

Conclusions:

  • CD97 negatively regulates LPS-induced NF-κB activation and TNF-α secretion in macrophages.
  • This suppression is partly mediated by the upregulation of PPAR-γ.
  • CD97 plays a crucial role in modulating inflammatory responses in macrophages.

More Related Videos

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.9K
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

69.1K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.3K
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

2.5K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.8K