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Proteolysis and cell death.
1Department of Pathology, University of Pittsburgh School of Medicine, PA 15261.
Revisiones Sobre Biologia Celular : RBC
|January 1, 1989
Summary
Selective cell death in L-cells involves protein accumulation and hyaline body formation, distinct from general necrosis. This process, characterized by increased proteolysis, can be prevented by cycloheximide.
Area of Science:
- Cell Biology
- Biochemistry
Background:
- Cell death mechanisms vary, with some involving significant protein degradation.
- Understanding selective cell death is crucial for distinguishing it from general necrosis.
Purpose of the Study:
- To investigate the characteristics of selective cell death in L-cells.
- To differentiate selective cell death from general necrosis biochemically and morphologically.
Main Methods:
- L-cells were labeled with 14C-leucine and 3H-thymidine.
- Selective cell death was induced using thymidine; general necrosis was induced by freeze-thawing or NaCN.
- Proteolysis and cell death were assessed in the presence of NH4Cl and cycloheximide.
Main Results:
- Selective cell death, unlike general necrosis, showed a lag phase followed by cellular enlargement and protein accumulation.
- Eosinophilic hyaline bodies formed and were released during selective cell death.
- Increased proteolysis was observed in selective cell death, inhibited by NH4Cl, while cell death and hyaline body formation were prevented by cycloheximide.
Conclusions:
- Selective cell death (apoptosis-type) in L-cells is a distinct process involving protein accumulation and hyaline body formation.
- Vacuolar proteolysis increases during selective cell death, potentially mimicking accelerated protein turnover.
- Cycloheximide effectively prevents selective cell death, hyaline body formation, and associated proteolysis.