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Probiotics

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Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...

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Related Experiment Video

Updated: Mar 23, 2026

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

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Tackling probiotic and gut microbiota functionality through proteomics.

Lorena Ruiz¹, Claudio Hidalgo², Aitor Blanco-Míguez³

¹Department of Nutrition, Food Science and Technology, Complutense University of Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain.

Journal of Proteomics

|March 23, 2016

Summary

Proteomics, the study of proteins, is crucial for understanding how probiotics function and benefit the host. This review highlights how proteomic techniques identify molecular mechanisms behind probiotic health benefits and gut interactions.

Keywords:

Gut microbiota Metaproteomics Probiotics Proteomics Stress adaptation

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Area of Science:

Microbiology
Biochemistry
Gastroenterology

Background:

Probiotics are live microorganisms conferring health benefits.
Gut microbiota imbalances are linked to diseases.
Understanding probiotic mechanisms has been challenging.

Purpose of the Study:

To review studies using proteomics to assess probiotic functionality.
To identify molecular players in probiotic mechanisms of action.

Main Methods:

Proteomics offers high-throughput protein identification.
Utilized for studying probiotic functionality and health benefits.
Applied to assess immunomodulatory activity and gut colonization.

Main Results:

Proteomics aids in understanding probiotic-host interactions.
Identifies markers for technological performance and stress adaptation.
Helps predict probiotic behavior in food and survival in the gut.

Conclusions:

Proteomics is vital for elucidating probiotic functions.
Advances in proteomic techniques will enhance knowledge of probiotics and gut commensals.
Essential for evidence-based selection of probiotic microorganisms.