Related Experiment Video
Updated: Mar 23, 2026

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
Published on: January 28, 2014
Comparison of blood microsampling with DBS and conventional blood collection techniques used in a midazolam biostudy
Sylvain Lachance1, Marie-Claude Théberge1, Guy Havard1
1inVentiv Health Clinical, 2500 Einstein, Quebec, QC G1P 0A2, Canada.
Background:
Quantitative DBS LC-MS/MS assay for midazolam was used to compare two sample collection techniques (venipuncture and finger prick) and the midazolam concentrations measured in plasma samples, DBS and dried plasma spots.
Methodology:
Midazolam was extracted from DBS cards and compared with whole blood collected from usual venipuncture. Dried plasma spots were also compared with plasma. The blood volume used as well as the temperature impact during the blood and plasma deposits was evaluated. Midazolam was administrated to six healthy subjects during a clinical trial to obtained blood and plasma samples for the statistical comparison.
Conclusion:
The method for midazolam using DBS was validated and showed an excellent performance. Excellent correlations were observed when the same collection procedures were used.
Related Concept Videos
Bioavailability Study Design: Single Versus Multiple Dose Studies
Measurement of Bioavailability: Pharmacodynamic Methods
Measurement of Bioavailability: Pharmacokinetic Methods
Bioavailability Study Design: Healthy Subjects Versus Patients
Drug Concentrations: Measurements
Plasma...
Analysis of Population Pharmacokinetic Data

