Molecular basis of 5-hydroxytryptophan synthesis in Saccharomyces cerevisiae
Jiantao Zhang1, Chaochen Wu2, Jiayuan Sheng2
1Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. xueyang@vt.edu and Department of Pharmacology & Toxicology, University of Arizona, Tucson, AZ 85721, USA.
Abstract:
We report for the first time that 5-hydroxytryptophan can be synthesized in Saccharomyces cerevisiae by heterologously expressing prokaryotic phenylalanine 4-hydroxylase or eukaryotic tryptophan 3/5-hydroxylase, together with enhanced synthesis of MH4 or BH4 cofactors. The innate DFR1 gene in the folate synthesis pathway was found to play pivotal roles in 5-hydroxytryptophan synthesis.
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