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Updated: Aug 6, 2026
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Protocol for programming DNA crystal morphology using concentration-modulated W/O/W double-emulsion microfluidic
Jia Liu1, Xugen Chen2, Lebing Wang3
1Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, China; Ningbo Cixi Institute of Biomedical Engineering, Ningbo, Zhejiang 315201, China.
Abstract:
Here, we present a protocol for generating concentration-modulated water-in-oil-in-water (W/O/W) double-emulsion droplets (DEDs) to program DNA crystal morphology. We describe assembly of a dual-inner-phase glass capillary microfluidic device, flow-rate-controlled co-encapsulation, osmotic shrinkage, and image-based morphology analysis. This protocol validates encapsulation predictability using methylene blue and enables aspect-ratio control of 2T crystals and directional morphology regulation in 13-mer systems. For complete information on the generation and use of this protocol, please refer to Chen et al.1.
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