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Updated: Sep 20, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
Published on: March 12, 2018
Protocol to establish endogenous knockins in Jurkat T-ALL cells using CRISPR-Cas9-mediated homology-directed repair
Joana R Costa1, Yang Li1, Sara Ahrabi1
1Department of Haematology, UCL Cancer Institute, University College London, London, UK.
Abstract:
Jurkat cells are a versatile cell-line model utilized across multiple areas of biology. Here, we present a protocol for inserting DNA sequences (knockins) at an endogenous locus using CRISPR-Cas9 technology via homology-directed repair (HDR). We describe steps for designing, constructing, and validating endogenous knockins in T-ALL Jurkat cells. This protocol has potential application in gene regulatory studies, cancer biology, HIV research, and T-cell signaling, among others. For complete details on the use and execution of this protocol, please refer to Costa JR et al.1.
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