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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
Antioxidant activities of Eryngium caucasicum inflorescence
S Motallebi Riekandeh1, M Mazandarani, M A Ebrahimzadeh
1Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran. zadeh20@gmail.com.
Objective:
Medicinal plants are good sources for discovery of safe and new antioxidants. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidants from Eryngium caucasicum.
Materials And Methods:
Ultrasonically assisted extraction, Soxhlet extraction and percolation method were evaluated for the extraction of antioxidants from E. caucasicum inflorescence. Antioxidant activities of extracts were evaluated with four different in vitro tests.
Results:
Soxhlet extract had higher total phenolic and flavonoid contents than other extracts. Soxhlet extract showed the highest activity in DPPH radical scavenging (IC50 = 83.1 ± 2.1 µg ml-1). Soxhlet extract showed the best activity in iron chelatory (IC50 = 272 ± 6.3 µg ml-1) followed by ultrasonic extract. Percolation extract showed higher NO radical scavenging (IC50 = 390 ± 11.4 µg ml-1) than other extracts. Extracts showed good reducing power (p > 0.05).
Conclusions:
The results obtained indicated that all three extraction methods especially Soxhlet method could effectively extract antioxidants from this plant.
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