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Updated: Mar 23, 2026
04:23
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Published on: April 28, 2019
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Actin Interacts with Dengue Virus 2 and 4 Envelope Proteins
Kunlakanya Jitoboam1, Narumon Phaonakrop2, Sirikwan Libsittikul1
1Institute of Molecular Biosciences, Mahidol University, Salaya campus, 25/25 Phuttamonton Sai 4, Salaya, Nakorn Pathom, Thailand.
Plos One
|March 25, 2016
Summary
Dengue virus (DENV) infection reduces cellular actin levels. The DENV envelope protein interacts with actin, a key finding for understanding viral mechanisms and developing treatments.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Dengue virus (DENV) is a major global health concern.
- The DENV envelope (E) protein is crucial for viral entry and is a primary target for immune responses.
- Limited information exists regarding cellular proteins interacting with DENV proteins.
Purpose of the Study:
- To identify cellular proteins that interact with the DENV envelope (E) protein.
- To investigate the impact of DENV infection on these interacting proteins and cellular structures.
Main Methods:
- Co-immunoprecipitation was used to identify DENV E protein interacting partners.
- Expression levels of identified proteins were analyzed during DENV infection.
- Gene transcription was assessed to understand protein level changes.
Main Results:
- Actin and actin-related proteins were identified as interacting partners with the DENV E protein.
- Actin was confirmed as a bona fide interacting partner for DENV serotypes 2 and 4.
- A decrease in actin and actin-related protein expression was observed during infection, independent of transcriptional regulation.
- No significant cytoskeletal reorganization was noted, suggesting cell-type specific interactions.
Conclusions:
- The DENV E protein interacts with cellular actin.
- DENV infection leads to non-transcriptionally regulated decreases in actin and related proteins.
- The interaction between actin and the DENV E protein may be cell-type specific.
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