Multiple forms of proteases of Bacteroides gingivalis and their cellular location

Protease of Bacteroides gingivalis ATCC 33277 was found in intracellular membrane-free, intracellular membrane-bound and extracellular fractions. The insoluble form of proteases was solubilized with a detergent. The elution patterns of proteases on gel filtration in each fraction were rather different. However, enzymatic properties of the proteases separated by gel filtration were very similar.