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Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

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Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
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Bone Remodeling01:40

Bone Remodeling

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Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Bone Cells and Tissue01:30

Bone Cells and Tissue

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Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
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Related Experiment Video

Updated: Mar 23, 2026

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
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A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

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[Transcriptome analysis and epigenetic analysis during osteoclastogenesis].

Shinya Nakamura1, Sakae Tanaka1

  • 1Graduate School of Medicine, Surgical Sciences, Orthopaedic Surgery, The University of Tokyo, Japan.

Clinical Calcium
|March 26, 2016
PubMed
Summary
This summary is machine-generated.

Receptor activator of nuclear factor-κB ligand (RANKL) is crucial for osteoclastogenesis. Recent epigenetic analyses, including histone modifications and regulatory element data, advance understanding of bone cell communication.

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Area of Science:

  • Bone Biology
  • Epigenetics
  • Cell Signaling

Context:

  • Osteoclastogenesis, the formation of bone-resorbing cells, is a critical process in bone remodeling.
  • The RANKL-RANK signaling pathway, identified in 1998, plays a pivotal role in this process.
  • Nfatc1 was later identified as a key downstream regulator of RANKL-RANK signaling.

Purpose:

  • To review recent advancements in epigenetic analysis of osteoclastogenesis.
  • To highlight the application of new technologies like big data analysis in this field.
  • To explore the potential of epigenetic data in understanding cell-cell crosstalk in bone.

Summary:

  • Epigenetic analysis methods, including histone modification (H3K4me3, H3K27me3) and regulatory element profiling (DNase-seq, FAIRE-seq), are increasingly applied to study osteoclastogenesis.
  • These advanced techniques provide deeper insights into the molecular mechanisms regulating bone cell differentiation and function.
  • Recent studies utilize these epigenetic datasets to investigate the complex interactions within the bone microenvironment.

Impact:

  • These epigenetic insights are expected to elucidate the crosstalk between osteoclasts and other bone cells, such as osteoblasts, osteocytes, and chondrocytes.
  • This understanding can pave the way for novel therapeutic strategies targeting bone diseases.
  • Advancements in epigenetic analysis contribute to a more comprehensive understanding of skeletal biology and disease pathogenesis.