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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Small molecules that target phosphorylation dependent protein-protein interaction.

Nobumoto Watanabe¹, Hiroyuki Osada²

¹Bio-Active Compounds Discovery Research Unit, RIKEN Center for Sustainable Resource Science, Wako, Saitama 351-0198, Japan; Bio-Probe Research Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama 351-0198, Japan.

Bioorganic & Medicinal Chemistry

|March 28, 2016

Summary

Small molecules targeting phosphorylation-dependent protein interactions offer cancer treatment potential. This review highlights modulators for 14-3-3, Plk1 polo box, and SCF ubiquitin ligase F-box proteins.

Keywords:

Protein phosphorylation Protein–protein interaction Small molecule

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Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Area of Science:

Molecular Biology
Biochemistry
Pharmacology

Background:

Protein-protein interactions are crucial for signal transduction pathways.
Phosphorylation frequently mediates these interactions, altering protein function.
Dysregulated signaling contributes to diseases like cancer.

Purpose of the Study:

To review small molecules that modulate phosphorylation-dependent protein-protein interactions.
To focus on three key interaction modules: 14-3-3, Plk1 polo box domain, and F-box proteins.
To introduce a novel screening system for identifying such modulators.

Main Methods:

Literature review of small molecules targeting specific protein-protein interaction modules.
Description of an original high-throughput screening system.
Analysis of the role of these modules in disease pathogenesis.

Main Results:

Identification and summary of existing small molecules that modulate 14-3-3, Plk1 polo box, and F-box protein interactions.
Demonstration of the utility of the developed screening system for discovering novel modulators.
Emphasis on the therapeutic potential of these small molecules.

Conclusions:

Small molecules targeting phosphorylation-dependent protein interactions are promising therapeutic agents.
The reviewed modules (14-3-3, Plk1 polo box, F-box) are critical nodes in signaling pathways.
The developed screening system provides a valuable tool for future drug discovery in oncology and other diseases.