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Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
Published on: January 7, 2019
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A Human Amnion-Derived Extracellular Matrix-Coated Cell-Free Scaffold for Cartilage Repair: In Vitro and In Vivo
Makiko Nogami1, Tomoatsu Kimura1, Shoji Seki1
11 Department of Orthopaedic Surgery, Faculty of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama, Japan .
Tissue Engineering. Part A
|March 29, 2016
Summary
A novel scaffold combining human amniotic mesenchymal cell-derived extracellular matrix (ECM) with polylactic-co-glycolic acid (PLGA) promotes cartilage repair. This cell-free ECM-PLGA scaffold supports mesenchymal stem cell growth and facilitates hyaline cartilage regeneration in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Extracellular matrix (ECM) from human amniotic mesenchymal cells (HAMs) exhibits diverse biological functions.
- Developing advanced biomaterials is crucial for effective tissue regeneration therapies.
Purpose of the Study:
- To create and characterize a novel HAM-derived ECM-coated polylactic-co-glycolic acid (ECM-PLGA) scaffold.
- To evaluate the scaffold's properties for mesenchymal stem cell (MSC) support.
- To investigate its potential as a cell-free therapeutic for cartilage repair.
Main Methods:
- ECM-PLGA scaffolds were fabricated by seeding HAMs on PLGA, followed by decellularization.
- In vitro studies assessed MSC attachment, proliferation, and differentiation markers (collagen II mRNA).
- In vivo studies involved implanting cell-free ECM-PLGA scaffolds into rat knee osteochondral defects and histological analysis at multiple time points.
Main Results:
- The ECM-PLGA scaffold composition mimicked natural amniotic stroma, including collagen type I, fibronectin, hyaluronic acid, and chondroitin sulfates.
- The scaffold demonstrated superior MSC attachment and proliferation.
- In vivo, ECM-PLGA implantation led to gradual tissue regeneration and significant hyaline cartilage repair compared to controls.
Conclusions:
- The cell-free ECM-PLGA scaffold provides a conducive environment for MSCs.
- This biomaterial effectively promotes cartilage repair in an in vivo model.
- ECM-PLGA represents a promising "ready-made" therapeutic agent for cartilage regeneration.
