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Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
Published on: January 18, 2017
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Light sheet microscopy and live imaging of plants
Béatrice Berthet1, Alexis Maizel1
1Center for Organismal Studies (COS), University of Heidelberg, Heidelberg, Germany.
Journal of Microscopy
|March 29, 2016
Summary
Light sheet fluorescence microscopy (LSFM) enables fast, low-phototoxicity live imaging for plants. This review details LSFM methods and applications, addressing its underutilization in plant biology.
Area of Science:
- Plant Biology
- Microscopy
- Biotechnology
Background:
- Light sheet fluorescence microscopy (LSFM) is a powerful technique for live imaging.
- LSFM offers optical sectioning, fast acquisition, and low phototoxicity, ideal for dynamic biological processes.
- Despite its advantages, LSFM is underutilized in plant science.
Purpose of the Study:
- To highlight challenges in live plant imaging.
- To describe key steps for applying LSFM to live plant samples.
- To provide an overview of LSFM applications in plant biology.
Main Methods:
- Review of existing literature on LSFM in plant biology.
- Description of LSFM principles and instrumentation relevant to plants.
- Analysis of challenges and solutions for plant sample preparation and imaging.
Main Results:
- LSFM provides high-resolution 3D imaging of plant samples over time.
- Key parameters for successful LSFM in plants include illumination control and detection strategies.
- Successful applications range from subcellular dynamics to whole-organ development.
Conclusions:
- LSFM has significant potential to advance plant science research.
- Overcoming current challenges will increase LSFM adoption in plant biology.
- Further development and application of LSFM will yield new insights into plant life.
