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Updated: Mar 23, 2026
A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
Published on: April 12, 2017
Oxygen saturation monitoring using resonance Raman spectroscopy
Ivo Torres Filho1, Nguyen M Nguyen2, Rizwan Jivani2
1Department of Anesthesiology, Virginia Commonwealth University and the Virginia Commonwealth University Reanimation Engineering Science Center, Richmond, Virginia; Department of Emergency Medicine, Virginia Commonwealth University and the Virginia Commonwealth University Reanimation Engineering Science Center, Richmond, Virginia; Damage Control Resuscitation, U.S. Army Institute of Surgical Research, Fort Sam Houston, Texas.
Background:
The knowledge of hemoglobin oxygen saturation (SO2) and tissue oxygenation is critical to identify the presence of shock and therapeutic options. The resonance vibrational enhancement of hemoglobin allows measurement of oxy- and deoxy species of hemoglobin and resonance Raman spectroscopy (RRS-StO2) has been successfully used to measure aggregate microvascular oxygenation. We tested the hypothesis that noninvasive oxygen saturation measured by RRS-StO2 could serve as surrogate of systemic central venous SO2.
Methods:
In anesthetized rats, measurements of RRS-StO2 made in oral mucosa, skin, muscle, and liver were compared with measurements of central venous SO2 using traditional multi-wavelength oximetry. Various oxygenation levels were obtained using a stepwise hemorrhage while over 100 paired blood samples and Raman-based measurements were performed. The relationships between RRS-StO2 and clinically important systemic blood parameters were also evaluated. RRS-StO2 measurements were made in 3-mm diameter tissue areas using a microvascular oximeter and a handheld probe.
Results:
Significant correlations were found between venous SO2 and RRS-StO2 measurements made in the oral mucosa (r = 0.913, P < 0.001), skin (r = 0.499, P < 0.01), and liver (r = 0.611, P < 0.05). The mean difference between sublingual RRS-StO2 and blood sample SO2 values was 5.4 ± 1.6%. Sublingual RRS-StO2 also correlated with lactate (r = 0.909, P < 0.01), potassium (r = 0.757, P < 0.01), and pH (r = 0.703, P < 0.05).
Conclusions:
Raman-based oxygen saturation is a promising technique for the noninvasive evaluation of oxygenation in skin, thin tissues, and solid organs. Under certain conditions, sublingual RRS-StO2 measurements correlate with central venous SO2.
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