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Three-Dimensional 3D Tumor Spheroid Invasion Assay
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Digital microfluidics for spheroid-based invasion assays.

Brian F Bender1, Andrew P Aijian1, Robin L Garrell2

  • 1Bioengineering Department, University of California, Los Angeles, CA 90095-1600, USA. garrell@chem.ucla.edu.

Lab on a Chip
|March 30, 2016
PubMed
Summary
This summary is machine-generated.

A novel digital microfluidic (DμF) platform automates 3D cell invasion assays using multicellular spheroids. This DμF system enhances accessibility for studying tissue microenvironments and cell migration in research.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cell invasion is crucial for tissue development and disease, but traditional 2D assays lack in vivo relevance.
  • Multicellular spheroids offer a 3D model mimicking tissue microenvironments for studying cell invasion.
  • Current spheroid invasion assays are often labor-intensive or require expensive automation.

Purpose of the Study:

  • To develop and validate a digital microfluidic (DμF) platform for automated spheroid-based cell invasion assays.
  • To create a more physiologically relevant 3D in vitro model for studying cell migration and tissue microenvironments.
  • To assess the platform's capability in manipulating collagen gels and delivering migration-modulating agents.

Main Methods:

  • Formation of multicellular spheroids using the hanging drop method on a DμF device.
  • Encapsulation of spheroids within collagen gels (up to 4 mg mL(-1)) on the DμF platform.
  • Automated delivery of exogenous agents to modulate spheroid migration and invasion assays.

Main Results:

  • Human fibroblast spheroids demonstrated invasion into collagen gels on the DμF platform.
  • Exogenous agents could enhance or inhibit fibroblast invasion, validating the platform's control.
  • Exposure to colon cancer spheroid secretions significantly increased fibroblast invasion (>100%), mimicking cancer-associated fibroblast behavior.

Conclusions:

  • The DμF platform successfully automates spheroid formation, encapsulation, and invasion assays, closely mimicking the tissue microenvironment.
  • This technology provides a more accessible and physiologically relevant alternative to traditional 2D cell invasion models.
  • The DμF system has broad potential for advancing automated 3D cell-based assays in life sciences research.