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Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae
Ana P Silva1, Isabel Faria-Ramos2, Elisabete Ricardo2
1Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal atsilva@med.up.pt.
Abstract:
A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.
