Related Experiment Video
Updated: Mar 23, 2026
09:21
Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging
Published on: June 15, 2020
3.6K
Effect of Genotype and Environment on Salvia miltiorrhiza Roots Using LC/MS-Based Metabolomics.
Qi Zhao1, Zhenqiao Song2, Xinsheng Fang3
1State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian 271018, China. sdauzhaoq@163.com.
Molecules (Basel, Switzerland)
|March 30, 2016
Summary
This study analyzed the metabolic profiles of Salvia miltiorrhiza roots, revealing significant variations based on location and genotype. These findings identify specific metabolic markers for S. miltiorrhiza, aiding in quality control and authentication.
Area of Science:
- Metabolomics
- Phytochemistry
- Pharmacognosy
Background:
- Salvia miltiorrhiza (S. miltiorrhiza) is a vital herbal medicine for cardiovascular and cerebrovascular diseases.
- Limited systematic metabolomic studies exist for S. miltiorrhiza roots.
- Understanding metabolic variations is crucial for its medicinal applications.
Purpose of the Study:
- To systematically characterize the metabolic profiles of S. miltiorrhiza roots.
- To investigate the impact of geographical location and genetic variations on secondary metabolites.
- To identify potential biomarkers for S. miltiorrhiza discrimination.
Main Methods:
- High-pressure liquid chromatography mass spectrometry (LC/MS) was employed for metabolic profiling.
- Multivariate statistical analyses, including Principal Component Analysis (PCA) and Orthogonal Projection to Latent Structure with Discriminant Analysis (OPLS-DA), were utilized.
- Metabolic profiles of S. miltiorrhiza from different locations and genotypes were compared.
Main Results:
- A total of 40 bioactive constituents were putatively identified in S. miltiorrhiza root samples.
- Significant metabonomic differences were observed between S. miltiorrhiza samples from different locations and genotypes.
- 16 and 14 secondary metabolites were identified as potential location-specific and genotype-specific markers, respectively.
Conclusions:
- LC/MS profiling effectively discriminates S. miltiorrhiza samples based on genotype and location.
- The identified metabolic markers can aid in the quality control and authentication of S. miltiorrhiza.
- This study provides a foundation for further research into S. miltiorrhiza's bioactive constituents and their variations.
