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U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

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Towards microfluidic sperm refinement: impedance-based analysis and sorting of sperm cells.

B de Wagenaar¹, S Dekker¹, H L de Boer¹

¹BIOS Lab on a Chip Group, MESA+ and MIRA Institutes, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. b.dewagenaar@utwente.nl, l.i.segerink@utwente.nl.

|March 31, 2016

Summary

This study introduces a novel microfluidic device for label-free sperm sorting, improving artificial insemination efficiency. The technology non-invasively separates normal sperm cells, reducing waste and enhancing fertilization potential in livestock.

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Last Updated: Mar 23, 2026

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Published on: November 23, 2015

Area of Science:

Reproductive biology and microfluidics.
Biotechnology and biosensing.

Background:

High-quality semen is crucial for livestock artificial insemination.
Morphological sperm defects, like cytoplasmic droplets, negatively impact fertilization.
Current methods discard up to 80% of semen samples, losing normal sperm.

Purpose of the Study:

To develop a label-free, non-invasive microfluidic system for detecting and sorting morphologically normal sperm cells.
To refine rejected semen samples, preserving valuable normal sperm.

Main Methods:

Utilized differential impedance measurements to detect cytoplasmic droplets on sperm flagella.
Developed a LabVIEW-based system for label-free sperm sorting using impedance and dielectrophoresis.
Performed proof-of-concept experiments with sperm cells and 3 μm beads.

Main Results:

Differential impedance measurements accurately detected cytoplasmic droplets with an AUC of 0.85.
Successfully sorted sperm cells and beads label-free using impedance detection and dielectrophoresis.
Demonstrated the feasibility of microfluidic sperm refinement.

Conclusions:

The developed microfluidic setup shows promise for label-free, non-invasive sperm cell detection and sorting.
This technology can potentially reduce semen waste and improve artificial insemination outcomes.
Further research aims to optimize microfluidic sperm refinement for practical applications.