Related Experiment Video
Updated: Mar 23, 2026
Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
Expression of Basophil Activation Markers in Pediatric Asthma
Masooma Abdullahi1, Reza Ranjbaran, Soheaila Alyasin
1Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Background:
Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms.
Objective:
To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma.
Methods:
Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR.
Results:
Patients expressed significantly higher levels of CD203c than healthy controls (p=0.01). Although there was an increase in the transcription level of ST2L gene in patients, the results were not statistically significant compared to those obtained from the healthy controls (p>0.05). A Specificity of 60% and a sensitivity of 73% were found using ROC curve for CD203c expression. Patients with positive family history of asthma exhibited more CD203c and ST2L expression (p<0.05).
Conclusion:
It is proposed that determining CD203c expression by real time PCR may be an effective technique for diagnosis of pediatric asthma.
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