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Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure
Cheryl J Ashworth1, Susan O George2, Charis O Hogg2
1The Roslin Institute and Royal (Dick) School of Veterinary StudiesUniversity of Edinburgh, Edinburgh, UK cheryl.ashworth@roslin.ed.ac.uk.
Insights
Maternal prenatal stress in rats altered offspring reproductive development. Prenatally stressed males showed enhanced reproductive axis development, suggesting males are more sensitive to maternal stress effects.
Area of Science:
- Reproductive biology
- Endocrinology
- Developmental neuroscience
Background:
- Maternal stress during pregnancy can impact offspring physiology.
- The effects of prenatal stress on the reproductive axis and adrenal glands are not fully understood.
Purpose of the Study:
- To investigate the impact of prenatal social stress on the reproductive axis and adrenal gland structure in adult male and female rat offspring.
- To determine if prenatal stress differentially affects male and female reproductive development.
Main Methods:
- Pregnant rats were exposed to social stress or control conditions.
- Offspring (male and female) were assessed at 11-12 weeks for reproductive organ weights, histology, hormone levels, and gene expression.
- Anogenital distance was measured in offspring.
Main Results:
- Prenatal stress did not affect offspring body, adrenal, or gonad weights.
- Female offspring showed a trend towards increased primary follicles and ovarian aromatase expression.
- Male offspring exhibited longer anogenital distances and higher plasma FSH concentrations.
Conclusions:
- The male reproductive axis appears more sensitive to prenatal maternal stress.
- Prenatal stress may enhance certain aspects of male reproductive development.
- Further research is needed to elucidate the mechanisms underlying these sex-specific effects.
Abstract:
Social stress during pregnancy has profound effects on offspring physiology. This study examined whether an ethologically relevant social stress during late pregnancy in rats alters the reproductive axis and adrenal gland structure in post-pubertal male and female offspring. Prenatally stressed (PNS) pregnant rats (n=9) were exposed to an unfamiliar lactating rat for 10 min/day from day 16 to 20 of pregnancy inclusive, whereas control pregnant rats (n=9) remained in their home cages. Gonads, adrenal glands and blood samples were obtained from one female and one male from each litter at 11 to 12-weeks of age. Anogenital distance was measured. There was no treatment effect on body, adrenal or gonad weight at 11-12 weeks. PNS did not affect the number of primordial, secondary or tertiary ovarian follicles, numbers of corpora lutea or ovarian FSH receptor expression. There was an indication that PNS females had more primary follicles and greater ovarian aromatase expression compared with control females (both P=0.09). PNS males had longer anogenital distances (0.01±0.0 cm/g vs 0.008±0.00 cm/g; P=0.007) and higher plasma FSH concentrations (0.05 ng/mL vs 0.006 ng/mL; s.e.d.=0.023; P=0.043) compared with control males. There were no treatment effects on the number of Sertoli cells or seminiferous tubules, seminiferous tubule area, plasma testosterone concentration or testis expression of aromatase, FSH receptor or androgen receptor. PNS did not affect adrenal size. These data suggest that the developing male reproductive axis is more sensitive to maternal stress and that PNS may enhance aspects of male reproductive development.
