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Fluorescent H-Aggregates Hosted by a Charged Cyclodextrin Cavity.

Niyati H Mudliar1,2, Prabhat K Singh3

  • 1Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400 085, India.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|March 31, 2016
PubMed
Summary

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Articles linked to this work by shared authors, journal, and citation graph.

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Reply to "Comment on the 'YOPRO-1: A Cyanine-Based Molecular Rotor Probe for Amyloid Fibril Detection'".

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Ultrafast Two-Dimensional Infrared Spectroscopy and Molecular Dynamics Simulations Reveal Slow Water Dynamics in Sulfobutylether-β-Cyclodextrin.

The journal of physical chemistry. B·2025

A negatively charged cyclodextrin derivative unexpectedly caused aggregation of Thioflavin-T, a dye used for amyloid detection. This resulted in the formation of rare, emissive H-type dye aggregates.

Area of Science:

  • Supramolecular Chemistry
  • Biophysical Chemistry
  • Materials Science

Background:

  • Macrocyclic host molecules, like cyclodextrins, typically prevent guest molecule aggregation via inclusion complexation.
  • Amyloid sensing dyes, such as Thioflavin-T, are crucial for detecting protein aggregates associated with neurodegenerative diseases.

Purpose of the Study:

  • To investigate the interaction between a negatively charged cyclodextrin derivative and Thioflavin-T.
  • To explore the formation of novel aggregate structures and their optical properties.

Main Methods:

  • Synthesis and characterization of a negatively charged beta-cyclodextrin derivative.
  • Spectroscopic analysis (UV-Vis, fluorescence) of Thioflavin-T in the presence of the cyclodextrin derivative.
  • Microscopy techniques to visualize aggregate formation.
Keywords:
H-aggregatemolecular rotorself-assemblystimulus-responsivethioflavin-T

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Main Results:

  • The negatively charged beta-cyclodextrin derivative induced the aggregation of Thioflavin-T, contrary to the typical behavior of host molecules.
  • Unprecedented formation of emissive H-type aggregates of Thioflavin-T was observed.
  • The study characterized the structural and photophysical properties of these rarely observed aggregates.

Conclusions:

  • Anionic cyclodextrins can promote, rather than inhibit, the aggregation of specific guest molecules like Thioflavin-T.
  • This finding opens new avenues for controlling dye aggregation and developing novel amyloid sensing strategies.