Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental observation of counter-intuitive features of photonic bunching.

Light, science & applications·2026
Same author

Vacuolar and ER-Ca2+-ATPases regulate calcium dynamics during pollen tube growth in Arabidopsis thaliana.

Plant physiology·2026
Same author

New Perspectives Provided by Merging Computed Tomographic Scanning and Electroanatomical Mapping of Koch's Pyramid.

Journal of cardiovascular development and disease·2026
Same author

Experimental data reuploading with provable enhanced learning capabilities.

Science advances·2026
Same author

Amplitude- and Phase-Programmable Dual-Color Photonic Chip for High-Contrast Structured Illumination Microscopy.

ACS photonics·2026
Same author

MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.

Science advances·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Mar 23, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K

Selective plane illumination microscopy on a chip.

Petra Paiè1, Francesca Bragheri2, Andrea Bassi3

  • 1Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. andreabassi@polimi.it and Istituto di Fotonica e Nanotecnologie, CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.

Lab on a Chip
|April 1, 2016
PubMed
Summary
This summary is machine-generated.

Selective plane illumination microscopy (SPIM) achieves high-resolution imaging but faces throughput limitations. This study introduces an integrated optofluidic device using laser micromachining for continuous-flow, high-throughput SPIM of multicellular spheroids.

More Related Videos

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

18.3K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K

Related Experiment Videos

Last Updated: Mar 23, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K
Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

18.3K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K

Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Optics

Background:

  • Selective plane illumination microscopy (SPIM) offers high spatiotemporal resolution for biological imaging.
  • Traditional SPIM methods are hindered by complex sample preparation and system alignment, limiting throughput.
  • Analyzing large multicellular spheroids requires advanced imaging techniques capable of subcellular resolution.

Purpose of the Study:

  • To develop an integrated optofluidic device for high-throughput SPIM.
  • To overcome the limitations of conventional SPIM sample preparation and alignment.
  • To enable continuous-flow imaging and 3D reconstruction of multicellular spheroids.

Main Methods:

  • Utilized femtosecond laser micromachining to fabricate an integrated optofluidic device.
  • Implemented continuous-flow imaging capabilities within the optofluidic system.
  • Integrated 3D reconstruction and high-throughput analysis functionalities.

Main Results:

  • Demonstrated continuous-flow imaging of large multicellular spheroids.
  • Achieved subcellular resolution in the imaging of spheroid structures.
  • Enabled high-throughput analysis, significantly improving the efficiency of SPIM.

Conclusions:

  • The developed optofluidic device enhances the throughput of selective plane illumination microscopy.
  • Femtosecond laser micromachining provides a viable method for creating advanced imaging devices.
  • This technology facilitates efficient, high-resolution analysis of complex biological samples like multicellular spheroids.