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ATP-sensitive K(+) channels (Kir6.1/SUR1) regulate gap junctional coupling in cochlear-supporting cells
Alexander Blödow1, Daniela Begandt2, Almke Bader2
1Department of Otolaryngology, Head and Neck Surgery and Communication Disorders, HELIOS-Klinikum Pirna, Pirna, Germany.
Pflugers Archiv : European Journal of Physiology
|April 1, 2016
Summary
Intracellular ATP is essential for maintaining gap junction coupling in cochlear Hensen cells. Its absence leads to uncoupling, suggesting ATP-sensitive potassium (KATP) channels regulate this process.
Area of Science:
- Auditory Neuroscience
- Cell Physiology
- Molecular Biology
Background:
- Gap junctions are crucial for electrical coupling in cochlear-supporting Hensen cells.
- The metabolic state of supporting cells influences cochlear function, but the underlying mechanisms are not fully understood.
Purpose of the Study:
- To investigate the role of intracellular ATP in regulating gap junction coupling in Hensen cells.
- To identify the molecular mechanisms linking ATP levels to gap junction function in the cochlea.
Main Methods:
- Double whole-cell patch-clamp recordings in Hensen cells.
- Pharmacological manipulation of intracellular ATP levels.
- Immunostaining of guinea pig cochlear tissue for KATP channel subunits.
Main Results:
- Absence of intracellular ATP caused progressive gap junction uncoupling in Hensen cells.
- ATP, but not its hydrolysis, was required to maintain conductance; non-hydrolyzable ATP analog (AMP-PNP) prevented uncoupling.
- External barium and glibenclamide mimicked ATP depletion effects, and KATP channel subunits (Kir6.1, SUR1) were detected in Hensen cells.
Conclusions:
- Intracellular ATP acts as a ligand to keep ATP-sensitive potassium (KATP) channels closed, thereby maintaining Hensen cell gap junction coupling.
- KATP channels in Hensen cells serve as a physiological link between cellular metabolism and potassium recycling in the organ of Corti.
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