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Published on: April 11, 2016
Polysaccharide extraction from Sphallerocarpus gracilis roots by response surface methodology
Tingting Ma1, Xiangyu Sun2, Chengrui Tian3
1College of Food Engineering an d Nutritional Science, Shaanxi Normal University, Xi'an 710062, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Ultrasonic-assisted extraction (UAE) is a superior method for isolating Sphallerocarpus gracilis root polysaccharides (SGRP) compared to hot-water extraction (HWE). UAE yields higher antioxidant activity with improved efficiency, making it a viable alternative.
Area of Science:
- Food Science
- Biotechnology
- Natural Product Chemistry
Background:
- Sphallerocarpus gracilis root polysaccharides (SGRP) are of interest for their potential health benefits.
- Optimizing extraction methods is crucial for maximizing yield and bioactivity.
- Conventional hot-water extraction (HWE) is widely used but may have limitations.
Purpose of the Study:
- To optimize and compare hot-water extraction (HWE) and ultrasonic-assisted extraction (UAE) for SGRP.
- To evaluate the antioxidant activities of SGRP obtained by different methods.
- To analyze the structural differences of extracted polysaccharides and residues.
Main Methods:
- Response surface methodology was employed to optimize UAE and HWE parameters.
- Extraction conditions optimized included temperature, time, liquid-solid ratio, and ultrasonic power (for UAE).
- Antioxidant activity assays and scanning electron microscopy (SEM) were used for characterization.
Main Results:
- Optimal UAE conditions: 81°C, 1.7h, 17ml/g, 300W, 3 cycles.
- Optimal HWE conditions: 93°C, 3.6h, 21ml/g, 3 cycles.
- UAE achieved higher yield, shorter extraction time, lower temperature, reduced solvent consumption, and superior antioxidant capacity.
Conclusions:
- Ultrasonic-assisted extraction (UAE) is more efficient and effective than HWE for SGRP extraction.
- UAE yields SGRP with enhanced antioxidant properties.
- UAE presents a promising alternative to conventional HWE for SGRP isolation.
